ROLE OF ANTI-MÜLLERIAN HORMONE (AMH) AND OTHER HORMONAL MARKERS IN THE BIOCHEMICAL ASSESSMENT OF FEMALE INFERTILITY

Abstract

INTRODUCTION:-Infertility is clinically defined as the failure to achieve a clinical
pregnancy after 12 months or more of regular unprotected sexual intercourse (World
Health Organization [WHO], 2020). Female infertility is a multifactorial condition
influenced by a broad spectrum of anatomical, genetic, immunological, and
endocrine variables. The biochemical evaluation of female infertility typically
encompasses a comprehensive analysis of ovulatory function, ovarian reserve, and
the presence of endocrine disorders.
AIM: -To evaluate serum AMH levels and it’s correlation with ovarian reserve &
infertility status.
MATERIALS AND METHODS:-This study is a observational cross-sectional
hospital-based study undertaken in the Department of Biochemistry & Obstetrics
Gynecology, Index medical College & Hospital, Indore MP from July 2021 to June
2024. Total number of 200 patients, include Women with infertility aged 18 – 40
presenting in Department of Obstetrics & Gynecology (OB/GYN). Five milliliter of
venous blood was collected, from each patient in plain tube. After centrifugation at
6000 rpm for 5 min. Biochemical Hormonal Parameters like serum LH, FSH, TSH,
Prolactin and serum AMH to be assessed in the Department of the Biochemistry.
RESULT:-. In the present study, A total of 100 fertile patients & 100 infertile
patients. On the basis of serum AMH level patients were divided into five groups and
maximum patients (45%) belonged to group 3 with serum AMH level between 9-
13ng/ml. AMH had an inverse relationship with BMI and obesity. Serum AMH level
showed a significant positive correlation with LH: FSH ratio (p <0.001) while there
was a significant negative correlation with FSH level (p = 0.011).
CONCLUSION: Female infertility is a challenge because of ever changing criteria
and its definition as infertile women have variable clinical presentation. AMH level
is consistently elevated in infertile patient as compared to controls. AMH is a useful
biochemical marker for diagnosis of infertility. It has a greater sensitivity and
specificity as compared to serum LH & LH: FSH ratio.